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DNA Sequencer
Oligo Series 1000 DNA Synthesizer The Beckman 1000M (Oligo 1000M DNA Synthesizer) is a fully automated synthesizer with a built-in Trityl monitor and extensive diagnostic functions to monitor the synthesis process and ensure the quality and purity of the synthesized oligonucleotides. It has the capability to synthesize eight oligonucleotides sequentially in a rapid and economical cycle time. Complete multi-column system with manual and miscellaneous accessories. Extensive diagnostic functions to monitor the synthesis process and ensure the quality and purity of the synthesized oligonucleotides. It has the capability to synthesize eight oligonucleotides sequentially in a rapid and economical cycle time. The unit is capable of providing custom gene synthesis, gene libraries and codon bias adjustments as well as modified DNA such as phosphorylated, biotinylated or fluorescent labeled oligonucleotides These units come in excellent like-new condition, and complete with cables, cords and manual. Buy this item fully refurbished with our one year warranty for $9950.00USD OR Buy it working as is for $6950.00USD Reagents and columns available at extra cost. These can be purchased directly for Beckman Coulter. Here are some of them below. Reactor Columns, 1/Pack Single pore-size glass column supports with the first nucleotide covalently attached. Column threads directly onto the Oligo Series Synthesizers and are guaranteed to be suitable for the synthesis of both short and long oligonucleotides. Reactor Columns, 30 nmole, 1/Pack Part No. Description 337719 Reactor Columns, dA(Bz), 30 nmole 337793 Reactor Columns, dC(Ac), 30 nmole 337721 Reactor Columns, dG(iBu), 30 nmole 337722 Reactor Columns, T, 30 nmole Reactor Columns, 200 nmole, 1/Pack Part No. Description 337723 Reactor Columns, dA(Bz), 200 nmole 337795 Reactor Columns, dC(Ac), 200 nmole 337725 Reactor Columns, dG(iBu), 200 nmole 337726 Reactor Columns, T, 200 nmole Binary-Pak™ CED-Phosphoramidites The phosphoramidite and acetonitrile are premeasured and packaged in a ready-to-mix bottle configuration containing activated molecular sieves, to provide extended life of at least four weeks on the instrument. 0.25 gram Binary-Paks Part No. Description 337737 Binary-Pak™ CED-Phosphoramidites, dA(Bz), 0.25 gram/5 mL Acetonitrile 337790 Binary-Pak™ CED-Phosphoramidites, dC(Ac), 0.25 gram/5 mL Acetonitrile 337739 Binary-Pak™ CED-Phosphoramidites, dG(iBu), 0.25 gram/5 mL Acetonitrile 337740 Binary-Pak™ CED-Phosphoramidites, T, 0.25 gram/5 mL Acetonitrile 0.5 gram Binary-Paks Part No. Description 337743 Binary-Pak™ CED-Phosphoramidites, dA(Bz), 0.5 gram/10 mL Acetonitrile 337791 Binary-Pak™ CED-Phosphoramidites, dC(Ac), 0.5 gram/10 mL Acetonitrile 337745 Binary-Pak™ CED-Phosphoramidites, dG(iBu), 0.5 gram/10 mL Acetonitrile 337746 Binary-Pak™ CED-Phosphoramidites, T, 0.5 gram/10 mL Acetonitrile Mono-Pak™ CED-Phosphoramidites The phosphoramidite is packaged in a bottle containing activated molecular sieves for extended life of at least four weeks on the instrument. Requires acetonitrile (P/N 338161) for 0.5 g and 1.0 g sizes, respectively. 0.5 gram Mono-Paks Part No. Description 337704 Mono-Pak CED-Phosphoramidites, dA(Bz), 0.5 gram. 337705 Mono-Pak CED-Phosphoramidites, dC(Ac), 0.5 gram. 337706 Mono-Pak CED-Phosphoramidites, dG(iBu), 0.5 gram. 337707 Mono-Pak CED-Phosphoramidites, T, 0.5 gram. 1.0 gram Mono-Paks Part No. Description 337708 Mono-Pak CED-Phosphoramidites, dA(Bz), 1.0 gram. 337709 Mono-Pak CED-Phosphoramidites, dC(Ac), 1.0 gram. 337710 Mono-Pak CED-Phosphoramidites, dG(iBu), 1.0 gram. 337711 Mono-Pak CED-Phosphoramidites, T, 1.0 gram. Other Synthesis Reagents Part No. Description 338161 Base Diluent, Acetonitrile, 45 mL in 100-mL BottleUsed with Mono-Pak phosphoramidites at 50 mg/mL concentration. Includes activated molecular sieves to ensure dryness. <10 ppm water. 337731 DNA Synthesis Reagent KitRecommended for Oligo 1000 only. Includes: P/N 337732 Oxidize, 337733 DEblock, 337734 CAP 1, 337735 CAP 2, premeasured for simultaneous depletion and replenishment when used with the Oligo 1000. 338284 Activate Used to convert the stable phosphoramidite into reactive species (0.5 M Tetrazole/ Acentonitrile). 338293 Oxidizer Used to oxidize the phosphorous on each cycle (3% I2 in THF, pyridine, water). 338248 Deblock Used to remove the trityl protecting group on the phosphoramidite (3% Trichloroacetic Acid in Dichloromethane). 338282 Cap 1 (Cap A Replacement) Automatically combined with Cap 2 reagent during synthesis to terminate incomplete reaction sequences (10% Acetic Anhydride in Tetrahydrofuran, lutidine). 338295 Cap 2 (Cap B Replacement) Automatically combined with Cap 1 reagent during synthesis to terminate incomplete reaction sequences (17% N-methyl-Imidizole/Tetrahydrofuran). Post-Synthesis Reagents and Supplies Part No. Description 607819 UltraFAST Cleavage and Deprotection Kit (set of 80)Provides cleavage within 5 minutes at room temperature and deprotection within 5 minutes at 65°C. Includes 80 disposable syringes, 80 vials specially adapted with O-rings compatible with ammonia methylamine (AMA), and sufficient AMA to process 80 oligonucleotides synthesized at either 30 or 200 nmole scale or 40 oligonucleotides synthesized at 1,000 nmole scale. Replacement Parts and Supplies Part No. Description 964338 10 Liter Waste BottlePolyethylene, fluorinated, split- and puncture-resistant bottle to use with Oligo 1000M or with the Oligo 1000 in place of the 4 liter bottle. Meets DOT-2T (Department of Trans- portation) specificationsto transport most hazardous reagents. 607909 Shield, Acetonitrile - Plastic acetonitrile safety container. Requires P/N 607838 Cap. Replacement Parts Part No. Description 523198 RS-232 Cable - Straight-through, 25-pin serial cable (M-F) for X-Y plotter; 9 feet long. To link instrument to a personal computer for downloading sequences and for developing user-defined methods.
Protein Sequencer, ABI, Model 473A
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